The activities of green cell-free extracts of spinach leaves in performing photochemical transphosphorylation, photosynthetic phosphorylation, the HILL reaction and the light-induced formation of the endogenous reducing substance (ascorbic acid) were followed in parallel during the growth process of the plant. There was a certain parallelism between the development of the activities of photochemical transphosphorylation, of photo-synthetic phosphorylation and of the HILL reaction, activities being low in the earlier stage of growth, reaching a maximum just before efflorescence, and showing thereafter a more or less sharp decline. The activity in the light-induced formation of endogenous reducing substance was undetectable for the first 35 day-period of growth, reached a maximum about one week earlier than the other three activities, and again disappeared after 60 days of growth.
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