A comparative study of initiation and development of embryogenic callus from haploid embryos of several barley cultivars. II. Cytophotometry of embryos and callus

摘要

Cells of haploid barley embryos (Hordeum vulgareL. 'Bruce', 'Perth', and 'Klages') and callus cells originating from embryos were cytophotometrically examined to determine the ploidy level. Specific embryo tissues regularly exhibited predictable ploidies and smaller embryos had a higher percentage of haploid cells than larger embryos of the same age. The predominantly haploid cells of the scutellar epidermis of the embryo initiated callus which generally, at least initially, was haploid. Monitoring of the ploidy evolution of these haploid calli showed that over a 6-month period each line exhibited its own unique rate of polyploidization, although lines of the same cultivar showed similar trends. Accumulation of cells at the diploid level was often a characteristic of these cultures.