Detection of IgE antibody againstCandida albicansenolase and its crossreactivity toSaccharomyces cerevisiaeenolase

摘要

SummaryCandida albicans46 kDa protein, a glycolytic enolase enzyme, is an important allergen of the yeast. The purpose of the study was to detect circulating IgE and IgG antibodies againstC. albicansenolase (CAE). We isolated CAE using sequential DEAE Sephacel and Pl 1 column chromatography from spheroptasts ofC. albicans, and delected IgE and IgG antibody against CAE by immunoblotting. Crossreactivity of enolose ofC. albicansandSaccharomyces cerevisiaewas also examined by immunoblotting and immunoblot inhibition test. Among 54 sera with positive IgE RAST toC. albicans, IgE antibody against CAE was detected in 20 sera (37) and IgG antibody in 27 sera (50). The allergenic potency of CAE was confirmed using a skin‐prick test in three patients. Simultaneous IgE binding toS. cerevisiaeenolase was only observed in four out of 20 sera reacting to CAE. Pre‐treatment of sera with CAE completely inhibited IgE binding toS. cerevisiaeenolase. Whereas the latter only partially inhibited IgE binding to CAE. These results suggest that CAE shares some crossreacting epitopes withS. cerevisiaeenolase, representing minor components of CAE but dominant segments ofS. cerevisiaeenol