The Effect of Various Adjuvant Regimens and of Nematode Infection on the Reaginic Antibody Response to Egg-Albumin in the Rat

摘要

The role of some critical components of the potentiated reagin response to egg-albumin following nematode infection in the rat are examined. The phenomenon can conveniently be described in two stages: Stage 1. Following injection of the antigen reagin production must be initiated before infection with the parasite. To satisfy this precondition, egg-albumin requires to be injected with an adjuvant since no detectable reagin appears if the antigen alone is injected. Adjuvants found to be successful in this system were Bordetella pertussis, Freund’s complete or incomplete adjuvants, Corynebacterium parvum in water-oil emulsion and aluminium hydroxide gel. C. parvum in saline suspension proved ineffective. Stage 2.Nippostrongylus brasiliensis infection potentiates the circulating reagin titre up to 100 times. Attempts to potentiate by substituting B. pertussis for a parasite infection in stage 2 were unsuccessfu