The kanamycin resistance encoded by the neomycin phosphotransferase II gene (nptII) of transposon Tn5 is widely used in higher plant genetic transformation. The general process of plant transformation using nptII as a selectable marker gene, however, requires selecting kanamycin-resistant plants or tissues in culture. Even with the recently developed vacuum infiltration method for Arabidopsis thaliana transformation, the plant culture steps are not completely eliminated in selection for kanamycin-resistant transformants. This report assesses the feasibility of applying kanamycin as a spray in selecting for kanamycin-resistant Arabidopsis transformants grown in soil. Results showed that kanamycin-resistant transformants were effectively selected byspraying soil-grown Arabidopsis seedlings.
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