AbstractThe cladoceranDaphnia magna, the amphipodsHyalella aztecaandGammarus lacustris, the annelid wormLumbriculus variegatusand the fathead minnowPimephales promelaswere exposed to hexachlorobenzene (HCB) in flow‐through tests ranging from 2 to 68 d in duration to determine the effects of HCB on survival, tissue bioconcentration andHyalellaandLumbriculusgrowth and reproduction.No effects on survival, growth or reproduction were observed at concentrations of HCB up to saturation (5.0 μ/L) or at tissue HCB concentrations of up to 223 μg/g. Tissue HCB concentrations increased with increasing water concentrations, and tissue bioconcentration values were generally similar. Rapid uptake and change of tissue HCB concentration with change in water concentration occurred, with rapid depuration when the animals were no longer exposed to HCB in the water column, and tissue HCB concentrations were correlated closely with HCB water concentrati
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