Effect of deficiency of certain micronutrients on the activity of aldolase in radish plants grown in sand culture

摘要

Deficiencies of each of the micronutrients, iron, zinc, copper, and manganese, decreased the activity of FDP-aldolase (D-fructose-1,6-biphosphateD-glyceraldehyde-3-phosphate-lyase (EC 4.1.2.13)) in leaves of radish (Raphanus sativusL. var. All Season). The depression in the enzyme activity was overcome when deficient plants were supplied normal levels of the micronutrient in short supply. The depression in the enzyme activity could not be attributed to decreased protein levels in the deficient plants. Polyacrylamide gel electrophoresis revealed only one form of FDP-aldolase in All Season radish leaves. Deficiencies of iron, zinc, copper, or manganese resulted in the appearance of two forms of aldolase.