Immunocytochemical detection of bone marrow‐invasive neuroblastoma cells

摘要

Abstract:We evaluated the utility of an immunocytochemical technique employing the commercially available anti‐CD56 monoclonal antibody, NKH 1. The utility and sensitivity of this technique in the detection of invasive neuroblastoma (NB) cells in the bone marrow were compared with those of Wright‐Giemsa staining. The correlation coefficient for the percent NB cells detected using Wright‐Giemsa staining with the percent NKH 1 immunoreactive cells was 0.78. In the analysis of specificity, this monoclonal antibody showed slight cross‐reactivity with normal bone marrow cells, including macrophages, lymphocytes and osteoblasts. In the evaluation of the sensitivity of the NKH 1 immunocytochemical technique, SK‐N‐DZ and SK‐N‐SH NB cell lines were added to morphologically normal bone marrow mononuclear cells from patients without NB to the final NB cell line at concentrations of 2, 1 and 0.1. NB cells at the final concentration of 0.1 could be detected by the immunocytochemical technique. We conclude that the NKH 1 immunocytochemical staining technique is useful in the detection of metastatic NB cells