Effects ofPorphyromonas gingivalisculture products on the morphology of peripheral blood polymorphonuclear leucocytes from periodontitis patients and healthy subjects

摘要

Abstract.The effects ofPorphyromonas gingivalisculture supernatant from strain W50, its avirulent variant (W50/BE1) and sterile BM culture medium on the morphological changes in polymorphonuclear leucocytes (PMN) from 10 patients with periodontitis (3 juvenile periodontitis, 7 rapidly progressive periodontitis) were compared with those from 10 healthy controls. Large non‐polar cells (>160 μm2) were increased in patients to 182.8 (p=0.0076) and 245.5 (p=0.0002) of control values with W50 and W50/BE1 supernatant respectively. In contrast, numbers of small non‐polar cells (<80 μm2) and polarised cells were decreased in the patient group. Patient/control ratios for small non‐polar cells were 30.3 (p=0.0007) and 33.6 (p=0.0005) with W50 and W50/BE1, respectively, and 38.2 (p=0.0147) for polarised cells exposed to W50 supernatant. The data indicate that PMN from patients with periodontitis differ significantly from those of healthy controls in the shape changes which occur after exposure toP. gingivalisculture supe