Characteristics of Different Rhesus Alloantibodies in Lymphocyte Dependent (K Cell) Cytotoxicity to Human Erythrocytes

摘要

Abstract.The property of rhesus alloantibodies to elicit antibody‐dependent, cell‐mediated cytotoxicity (ADCC) against target erythrocytes carrying various Rh genotypes was studied. The killer activity of normal peripheral lymphocytes on human erythrocyte target cells carrying the appropriate antigens elicited by alloantisera was measured by51Cr release at 18 h. There was no correlation between ADCC and antibodies directed to the antigens present on the surface of different genotypes of Rh‐positive red blood cells. The agglutinin titre of different Rh antibodies showed no correlation with the level of ADCC although the degree of cellular cytotoxicity was different with different anti‐D sera. Anti‐C + D + E antibody caused higher ADCC than anti‐C + D and the lowest cytotoxicity was observed with anti‐D and anti‐D + E. This raised the possibility that ADCC was elicited by antibodies directed to other specificities. K cell lysis of human red cells by human peripheral blood lymphocytesin vitrosuggests that a similar mechanism may operatein vitroin the destruction of erythrocytes coated by alloor