ses, a novel visible mutant of Arabidopsis thaliana related to anther development

摘要

Analysis of mutants and clone of genes is crucial for unraveling the mechanism of anther development. The lack of elongation of siliques in a novel shortened early-stage siliques (ses) mutant was caused by unsuccessful pollination because of aborted pollens. At stage 11, microspores of mutant ses appeared weakly stained and degenerated. At stage 14, mutant ses produced abnormal pollens, and the anther wall was collapsed and crumpled; furthermore, some cavities were also found under the epidermis. Mutant ses showed that the gene responsible for the defects plays a role in anther development. By mapping, mutant ses was narrowed into a 67-kb interval on chromosome 1 between CER448792 (2,000,541 bp) and CER464544 (2,067,844 bp). By using the sequence-based map of Arabidopsis genes with mutant phenotypes, ses was localized on the right of phenotype marker AT1g06150 and on the left of phenotype marker AT 1 g08060. The phenotyping and mapping data both pointed to the conclusion that ses was a novel mutant related to anther development. Sequencing showed that there was a point mutation in gene AT1G06710.1; thus, gene AT1G06710.1 is the most likely candidate gene responsible for the mutation.