Effect of Combined Treatment of Serum Containing Hepatitis B Virus with Beta‐Propiolactone and Ultraviolet Irradiation

摘要

AbstractThe effect of cold sterilization by β‐propiolactone (β‐PL) and ultraviolet (UV) irradiation of serum contaminated with infectious hepatitis B virus (HBV) was investigated in chimpanzees. Chimpanzees given 0.1 ml/kg of the undiluted HBV serum estimated to contain 107‐108CID50/ml developed acute hepatitis B infection 4 weeks after inoculation. Chimpanzees injected with the same undiluted hepatitis serum treated with β‐PLAJV developed hepatitis B infection 14 weeks later. Based on the published linear relationship between log dose of HBV and incubation period in chimpanzees this indicates a 106‐fold reduction in infectivity titer. Animals inoculated with the serum diluted 1:1,000 showed manifest hepatitis B infection 11 weeks later. Animals inoculated with serum diluted 1:1,000 and then cold sterilized with b#x030a;‐PL/U V showed no signs of hepatitis B infection. Sensitive proteins are not denaturated by β‐PL/UV c