Summary.Some of the factors contributing to the loss of viability of V5 contagious bovine pleuropneumonia vaccine in BVFOS medium during storage were investigated. Results showed that: 1, some types of rubber stoppers were highly toxic toMycoplasma mycoides; 2, loss of viability was greater when the vaccine was stored in smaller bottles and was related to the proportionately greater air space; 3, the addition of glycerol to BVFOS medium resulted in slightly better survival during storage at 37° but decreased survival at 4°; 4, small bottles can be filled to higher levels, and the air space reduced, if the mouth of the bottle is protected during incubation by a metal cover instead of a cotton wool plug. It is recommended that the amount of air space should not exceed 40 of the volume of the bottle, the vaccine should be stoppered with non‐toxic stoppers after incubation and the BVFOS medium should not be supplemented with glycerol. Vaccine prepared in this way should maintain a viable count of 107or more organisms/ml during storage at 4° for at least 12 w
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