A method which can be implemented in a routine organochlorine analysis laboratory equipped with a quadrupole GC/MS is presented for determination of tetrachloro-(T4-) to octachloro- (O8-) dibenzodioxins (CDDs) in animal tissues. Long chain-length biogenic compounds such as lipids are removed by gel-permeation chromatography (GPC), and the CDDs are separated from most interfering organochlorine compounds by a combination of alumina and Florisil chromatography. Recoveries from spiked samples were in the 87#x2013;97 range for T4CDDs-H7CDDs at the 5#x2013;100 ng/kg level except for 1,3,6,8- and 1,3,7,9,-T4CDD, which had variable losses in the alumina chromatography step (recoveries of 73#x2013;97). O8CDD recoveries depended on level, 80 at 200 ng/kg, 52#x2013;59 at 30#x2013;40 ng/kg. Precision of replicate analysis was generally in the 2#x2013;7 range. Positive interferences not completely removed by the method were identified as methoxychloro-biphenyls and methoxychloro-diphenylethers. Examples of the application of the method are given.
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