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>Survival of, and root colonization by, alginate-encapsulatedPseudomonas fluorescenscells following introduction into soil
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Survival of, and root colonization by, alginate-encapsulatedPseudomonas fluorescenscells following introduction into soil
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机译:Survival of, and root colonization by, alginate-encapsulatedPseudomonas fluorescenscells following introduction into soil
The survival ofPseudomonas fluorescenscells encapsulated in alginate beads and colonization of wheat roots was studied in soil microcosms inoculated with the cells in alginate beads of varying composition. Cells encapsulated in beads and introduced into a non-sterile loamy sand survived better than cells added directly to the same soil. A recovery/growth step for the bead-encapsulated cells was added before they were introduced into the soil, in an attempt to obtain optimal population levels in the soil. Further, bacterial populations that grew to the highest density in the beads subsequently showed the highest survival levels in soil. The addition of 3 skim milk, or 3 skim milk and 3 bentonite clay to all bead types consistently resulted in the highest survival of the encapsulated cells in soil. Root colonization byP. fluorescenswas generally not impaired by the encapsulation in alginate. One week after inoculation into the soil, encapsulated cells in the various bead types were able to colonize the wheat rhizoplane at high population levels, similar to or exceeding those found when free cells were inoculated. In a second root colonization experiment the wheat rhizoplane was also efficiently colonized 7 weeks after the inoculant cells had been introduced into the soil in different bead types. In both assays, the cells encapsulated in beads amended with skim milk plus bentonite clay showed the highest root colonization rates. It is clear, therefore, that alginate-mediated establishment of inoculants can improve inoculant effectiveness.
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