Pattern of Immunoreactivity After Reverse-Phase High Performance Liquid Chromatography of Ovine Lutropin at Different pH

摘要

Unlabelled and radioiodinated ovine lutropin (oLH) was fractionated using reverse-phase high pressure liquid chromatography, employing a 5 #x3BC;m Spherisorb C18column. Following chromatography of unlabelled oLH at pH 3.3, 4.3 and 5.3, all eluted fractions were tested by radioimmunoassay of oLH, oLH #x3B1; and oLH #x3B2;. Regardless of pH, the material corresponding to the first peak contained only #x3B1;-subunit immunoreactivity. With increasing pH, the oLH #x3B1;, oLH and oLH #x3B2; immunoreactivity separated into two groups of fractions. The retention times of the radioiodinated #x3B1;- and #x3B2;-subunits of oLH corresponded to those of the first (descending part) and last peaks from unlabelled oLH, respectively.