AbstractIsolation of symbiont and associated fungi from ectomycorrhizas of Sitka spruce from three Irish forest plantations is described. Frequent emergence of dark sterile mycelia andOidiodendronspp., predominantly O.maius, restricted the recovery of potential symbionts. Inhibition of the former by benomyl resulted in a sixfold increase in basidiomycetes isolated. Pure culture syntheses confirmed the ectomycorrhizal ability of potential symbiont isolates and demonstrated the parasitic nature of dark sterile mycelial types. Antagonism ofMycelium radicis atrovirens, Phytophthora cinnamomiandHeterobasidion annosumby O.maiusobserved in paired cultures supports the possible involvement of this species in biological control of conifer root pathogens.
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