Identification of EuropeanArmillariaspecies by restriction‐fragment‐length polymorphisms of ribosomal DNA

摘要

SummaryIdentification of EuropeanArmillariaspecies using ribosomal DNA (rDNA) restriction‐fragment‐length polymorphisms (RFLPs) was studied. A total of 44Armillariaisolates representing five European biological species were examined. Whole‐cell DNAs were digested with one or two (double digest) restriction endonucleases and probed with a cloned plasmid carrying one complete rDNA repeat copy ofSaccharomyces carlsbergensis. Applying the restriction endonucleaseBglII in combination with eitherEcoRI,BamHl orHindIII, it was possible to detect rDNA RFLPs allowing differentiation between all five EuropeanArmillariaspecies investigated in the study. The most conclusive results were obtained in the rDNA/AvaII RFLP pattern. All biological species showed uniqueAvaII banding patterns. Generally speaking, the interspecific similarities were around 42 and lower, indicating a distinct species separation.The analysis of rDNA RFLP patterns by a restriction‐enzyme‐rDNA‐probe combination (for instance,AvaII orBglII/HindIII; probe pMY 60) is a practical means of identifying EuropeanArmillariaspecies for further taxonomic, phylogenetic and host‐pathogen intera