The canine respiratory secretory immune response was studied by quantitative delivery of keyhole limpet hemocyanin (KLH) to the lower respiratory tract (LRT) and compared with intravenous administration of KLH. Respiratory secretions (RS) were standardized by IgA content and antibody against KLH studied by precipitin, passive hemagglutination and radioimmunoelectrophoretic (RIEP) techniques. Dogs immunized with KLH by either the respiratory or the intravenous route showed precipitating antibodies in serum but not RS. In these studies, aerosolized KLH resulted in IgA, IgG and IgM antibodies in RS and serum, and a comparatively higher antibody titer in RS than occurred following intravenous KLH.
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