Effect of cytochalasins on actin in dividing root tip cells ofAlliumandTriticum: A comparative immunocytochemical study

摘要

AbstractA collaborative effort was initiated to resolve differences in two recent papers on the effects of cytochalasins in root cells. While both studies reported similar effects on interphase cells (i.e., replacement of microfilaments by many small specks and rods), Palevitz(Cell Motil. Cytoskeleton9:283‐298, 1988) maintained that cytochalasins B and D induce actin aggregation at the poles of dividingAlliumroot cells at a concentration of 10 μM with rhodamine phalloidin as a reporter probe, whereas McCurdy and Gunning(Cell Motil. Cytoskeleton15:76‐87, 1990) could not find these aggregates following antiactin immunocytochemistry inTriticumroots treated with CB at 50 μM. Employing identical methods and materials in the same laboratory, we found that CD induces polar actin aggregates in dividing cells of both species. However, the aggregates inTriticumare smaller and occur less frequently than those inAllium.A similar pattern is seen wi