Cell survival and architectonic differentiation of the hypothalamic mamillary region of the newborn mouse in culture,

摘要

AbstractThe development of the hypothalamic mamillary region of the newborn mouse has been studied in 519 cultures. The nuclear groups of the mamillary configuration as well as the infundibular nucleus may survive for at least 50 days. The surviving neuronal groups undergo in culture a further cytological and structural organization. The presence of ependymal tissue at the surface of the substrate enhances ependymal‐glial proliferation and this in turn supports the survival and new growth of the nerve cells in the adjacent areas. On the other hand, degeneration of white matter, especially if it occurs between the substrate and the overlying neuronal groups, has a highly detrimental effect leading to a profound regional neuronal loss. The survival of neuronal groups, the new axonal growth and the preservation of the cellular architectonics, which make possible their identification in culture, indicate that the mamillary complex has a capacity for regeneration during its development in isolation. The mamillary region and the infundibular nucleus display different developmental patterns and different structural organizations of their cells and axons. As a result, the culture seems to be divided morphologically into two distinctly separate region