Microspore and pollen development in six male-sterile mutants ofArabidopsis thaliana

摘要

Five new recessive male-sterile mutants ofArabidopsis thalianawere isolated following seed mutagenesis by X-rays and ethyl methanesulfonate. The cytology of plants homozygous for themsYandmsWmutations suggested that pollen development in these lines became abnormal at or before meiosis. ThemsKmutation caused faulty timing of synthesis or turnover and distribution of callose. In plants homozygous for themsZmutation, pollen development failed at a late stage. In wild-type plants, the stamen filament elongated just prior to anther dehiscence. In contrast, in themsZmutant stamen elongation did not occur. Pollen inmsHhomozygotes was fertile, but anthers failed to dehisce. ThemsImutant of J.H. Van der Ween and P. Wirtz (1968. Euphytica 17: 371 ndash; 377) was included in the present study. Pollen development in this mutant failed shortly after microspore release from tetrads. Complementation tests confirmed that themsmutations were at different loci. Reduced transmission of certainmsgenes was observed.Key words:Arabidopsis thaliana, male sterile mutants, anther dehiscence, callose, inheritance.