TISSUE CULTURE OF PINUS MONTICOLA ON A CHEMICALLY DEFINED MEDIUM

摘要

Procedures for obtaining and maintaining axenic tissue cultures ofPinus monticolaare described. Western white pine tissue was cultured on a chemically defined medium containing calcium nitrate, magnesium sulfate, potassium phosphate (monobasic), ammonium sulfate, ferric sulfate, manganese sulfate, glucose, and one of three auxins, IAA, NAA, or 2,4-D. Addition of several B vitamins, amino acids, and kinetin increased growth on these media but were not required.