Purification, characterization and antimicrobial spectrum of a bacteriocin produced byPediococcus acidilactici

摘要

An antimicrobial peptide designated pediocin AcH was isolated fromPediococcus acidilacticistrain H. The pediocin AcH was purified by ion exchange chromatography. The molecular weight of pediocin AcH was determined by SDS‐PAGE to be about 2700 daltons. Pediocin AcH was sensitive to proteolytic enzymes, resistant to heat and organic solvents, and active over a wide range of pH. Pediocin AcH exhibited inhibition against several food spoilage bacteria and foodborne pathogens includingStaphylococcus aureus, Clostridium perfringensandListeria monocytogenes.It was bactericidal to sensitive cells and acted very rapidly. The bactericidal effect was not produced by either cell lysis or apparent loss of membrane permeabilit