SUMMARYEquations have been developed for calculation of the total quantity of pigments consisting of chlorophyllaand its derivatives (chlorophyllidea, pheophytina, and pheophorbidea) and of chlorophyllband its derivatives (chlorophyllideb, pheophytinb, and pheophorbideb) in a mixture of these pigments in diethyl ether. Calculations are based on conversion of the chlorophylls and chlorophyllides into the pheophytins and pheophorbides, respectively, by the addition of hydrochloric acid and reading absorbances at two wavelengths. After removal of chlorophyllides a andband pheophorbides a andbfrom the diethyl ether solution of the pigment mixture by 0.01NKOH, the quantity of each of the eight components listed above is estimated by indicated experimental and mathematical procedures.
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