α-Chymotrypsin covalently bound to silica, enzacryl AA, and enzacryl AH catalyzes peptide bond formation between N-protected dipeptide methyl esters and H-Leu-NH2with results similar to those with the free enzyme. The influence of water-miscible and water-immiscible cosolvents, of the supports, and of the structure of the substrates is shown to be of importance for the ease of the chymotrypsin-medicated coupling reactions. The best yields were obtained using biphasic aqueous-organic solvent mixtures, silica-bound chymotrypsin, and substrates with leucine in the P2-position. The yields of the syntheses are discussed in terms of the reactivity of substrates with similar structure in enzymatic hydrolyses. All the immobilized chymotrypsin preparations could be re-utilized successfully for further couplings
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