Revival of biocide‐treated spores ofBacillus subtilis

摘要

Spores ofBacillus subtilisNCTC 8236 were treated with biocides and then subjected to various revival procedures. Sodium hydroxide (optimum concentration 25 mmol 1−1) revived a small portion of glutaraldehyde‐treated spores but not of spores exposed to formaldehyde, polyvinylpyrrolidone‐iodine (PVP‐I), Lugol's iodine, sodium hypochlorite or sodium dichloroisocyanurate (NaDCC). Post‐treatment heat shock (at 70° or 80°C) increased the numbers of colony‐forming units (cfu) of formaldehyde‐injured spores. Coat‐extraction procedures had the greatest effect on iodine‐pretreated spores. The uptake of iodine and chlorine was more rapid and occurred to a greater extent with outgrowing, germinating and especially coat‐deficient spores than with m