Production ofBrassica napus × RaphanobrassicaHybrids by Embryo Rescue: An Attempt to Introduce Shattering Resistance intoB. napus

摘要

AbstractIntergeneric hybridization is one of the effective methods to broaden the genetic base of cultivated species.Raphanus sativus, a member of the subtribeRaphaninae, has very hard pods at threshing time. In an attempt to transfer shattering resistance toBrassica napus, Raphanobrassicawas used as the male parent in crosses withBrassica napus. Plantlets were obtained by embryo rescue and were further multipliedin vitroby micropropagation of nodal segments. Morphology, cytology and DNA analysis confirmed the hybrid nature of these plants. They were backcrosscd withBrassica napusand the progeny was raised. Plants of BC1and BC2generations showed wide variation in morphology, chromosome number and pollen fertility. Some of the plants showed up to 95 pollen fertility and resistance to shattering, indicating the potential for developingB. napuswith resistance to shattering.