The evaluation of a radioimmunoassay (RIA) for the monitoring of cyclosporine (CSA) that utilizes a monoclonal antibody specific for the parent compound is described. The assay exhibited good analytical recovery (95percnt;), precision (coefficient of variation 10percnt;), and linearity in plasma and whole blood specimens. The percentage cross reactivity of the major CSA metabolites M-17, M-1, and M-21 was 2percnt;. The RIA gave values that were on average indistinguishable from those obtained by high-pressure liquid chromatography in plasma and whole blood specimens obtained from 49 renal transplant recipients.
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