Abstract CD161 is used as a surrogate marker for Th17 cells, which are implicated in the pathogenesis of rheumatoid arthritis (RA). In this study, we evaluated the percentage, clinical significance, and CD98 and CD147 expression of CD4 + CD161 + T cells. The potential role of CD147 and CD98 in cyclophilin A-induced chemotaxis of CD4 + CD161 + T cells was analyzed. Thirty-seven RA patients, 15 paired synovial fluid (SF) of RA, and 22 healthy controls were recruited. The cell populations and surface expression of CD98 and CD147 were analyzed by flow cytometry. Spearman’s rank correlation coefficient and multiple linear regression were applied to calculate the correlations. Chemotaxis assay was used to investigate CD4 + CD161 + T cell migration. We found that the percentage of CD4 + CD161 + T cells and their expression of CD147 and CD98 in SF were higher than in the peripheral blood of RA patients. Percentage of SF CD4 + CD161 + T cells was positively correlated with 28-Joint Disease Activity Score (DAS28). CD147 monoclonal antibody (HAb18) attenuated the chemotactic ability of CD4 + CD161 + T cells. An increased CD4 + CD161 + T cell percentage and expression of CD147 and CD98 were shown in RA SF. Percentage of SF CD4 + CD161 + T cells can be used as a predictive marker of disease activity in RA. CD147 block significantly decreased the chemotactic index of CD4 + CD161 + cells induced by cyclophilin A (CypA). These results imply that the accumulation of CD4 + CD161 + T cells in SF and their high expression of CD147 may be associated with CypA-mediated chemotaxis and contribute to local inflammation in RA.
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机译：摘要 CD161被用作Th17细胞的替代标志物，Th17细胞与类风湿关节炎（RA）的发病机制有关。在这项研究中，我们评估了 CD4 + CD161 + T 细胞的百分比、临床意义以及 CD98 和 CD147 的表达。分析了CD147和CD98在亲环蛋白A诱导的CD4 + CD161 + T细胞趋化性中的潜在作用。招募了 37 例 RA 患者、15 例 RA 滑液 （SF） 配对和 22 例健康对照。采用流式细胞术分析CD98和CD147的细胞群和表面表达。采用Spearman秩相关系数和多元线性回归计算相关关系。趋化性测定用于研究 CD4 + CD161 + T 细胞迁移。我们发现 CD4 + CD161 + T 细胞的百分比及其在 SF 中 CD147 和 CD98 的表达高于 RA 患者外周血中的表达。SF CD4 + CD161 + T 细胞百分比与 28 关节疾病活动评分 （DAS28） 呈正相关。CD147 单克隆抗体 （HAb18） 减弱了 CD4 + CD161 + T 细胞的趋化能力。在 RA SF 中显示 CD4 + CD161 + T 细胞百分比增加以及 CD147 和 CD98 表达增加。 SF CD4 + CD161 + T 细胞的百分比可用作 RA 疾病活动的预测标志物。CD147阻断显著降低了亲环蛋白A（CypA）诱导的CD4+CD161+细胞的趋化指数。这些结果表明，SF 中 CD4 + CD161 + T 细胞的积累及其 CD147 的高表达可能与 CypA 介导的趋化性有关，并导致 RA 的局部炎症。
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