Visualization of the F9 antigen on sperm from normal and T/t‐complex mutants by immunoscanning electron microscopy

摘要

AbstractThe antigens defined by conventional syngeneic antiserum against F9 embryonal carcinoma cells were localized on mature sperm using immunolabeling and scanning electron microscopy. Labeling patterns were compared for normal (+ / +) mice and mice bearing recessive t‐haplotypes. The results showed that antigens detected by intact anti‐F9 antiserum are expressed similarly in all genotypes, except for sperm from mice bearing thet12‐haplotype where the frequency of labeled cells was reduced. Labeling with the IgM fraction of anti‐F9 antiserum was lower on sperm from all t‐genotypes examined, with sperm from + /t12males showing the most marked reduction. In all cases, the labeling patterns were similar, and included a labeling of the whole sperm head with complete anti‐F9 antiserum and a restriction of the label to the postacrosomal region when the IgM fracti