Yeast contaminants of micropropagated plant cultures

摘要

Leifert, C., Waites, W.M., Nicholas, J.R.Keetley, J.W. 1990. Yeast contaminants of micropropagated plant cultures.Journal of Applied Bacteriology69, 471–476.Of 36 yeast strains isolated from contaminated plant cultures 78 wereCandida,20Rhodotorulaand 2 wereCryptococcusspecies. Strains ofCandida guilliermon‐diirepresented 45 of all yeasts isolated. Yeasts grew rapidly on plant growth media with a pH of between 2.5 and 6.0 and decreased the medium pH to values of between 2.0 and 3.0.Candidayeasts growing on plant medium for 28 d metabolized about 75 of the sucrose and produced fermentation products such as ethanol and acetic acid. In contrast,Rhodotoruladid not produce ethanol or acetic acid and only metabolized about 20 of the sucrose. Possible sources of contamination are discus