Suspension cultures ofLotus corniculatusL., cv. Leo, were treated with the herbicides 2,4-D (2,4-dichlorophenoxyacetic acid) (10ndash;20ensp;ppm) or chlorsulfuron (2-chloro-N-(4-methoxy-6-methyl-1,3,5-triazin-2-yl)aminocarbonyl-benzenesulfonamide) (0.5ndash;1.0ensp;ppb) to select for either herbicide resistance or tolerance. Effects of the selection process on regeneration, pollen, chromosome number, and herbicide sensitivity of regenerates were evaluated. Plantlets regenerated from selective 2,4-D cultures exhibited lowin vitrorooting percentage, a high percentage of shrivelled pollen, and variation in chromosome number. The effect of 2,4-D was confounded, in some cases, with the effect of duration of culture period. A slight degree of tolerance was observed in one of the selections to chlorsulfuron at 10 to 20ensp;gensp;haminus;1. No sign of tolerance to 2,4-D was observed at 1.0ensp;kgensp;haminus;1.
展开▼
