Vanadate inhibition of mitochondrial respiration and H+ATPase activity inSaccharomyces cerevisiae

摘要

AbstractThe effects of vandate on mitochondrial respiration and H+ATPase activity inSaccharomyces cerevisiaewere studied. A 50 inhibition of oxygen uptake in isolated mitochondria was produced by 4·4 mM‐V2O5. Activity of H+ATPase in whole mitochondria was inhibited by 50 by 5·5 μM‐V2O5, in submitochondrial particles by 55 μM‐V2O5; and in the chloroform‐released H+ATPase by 0·5 mM‐V2O5. Vandate was also found to relieve growth inhibition caused by the mitochondrial H+ATPase inhibitors NN′‐decyclohexylcarbodiimide and oligomycin. These results imply that vanadate could affect mitochondrial respiration by interacting with the H+ATPa