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Autoradiographic localization of3H‐digoxin binding by neural cells in the medulla

机译:神经细胞在髓质中放射自显影定位 3H-地高辛结合

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AbstractThe purpose of this investigation was to localize binding sites for the cardiac glycoside digoxin in the medulla of the rat in vivo. Adult male Sprague‐Dawley rats were injected (IV) with3H‐digoxin and killed 30 minutes later. Autoradiographs of medullas showed evidence of3H‐digoxin binding to small‐ and medium‐sized neural cells in the regions of the nucleus solitarius, dorsal motor nucleus of the vagus, area postrema, and in the zone between the area postrema and the underlying neuropil. However, the parasympathetic preganglionic neurons of the dorsal motor nucleus were not labeled. The3H‐digoxin‐labeled cells in the medulla were located mainly in the commissural and medial portions of nucleus solitarius at the level of the area postrema. Animals injected with unlabeled digoxin followed by3H‐digoxin showed reduced binding of radioactivity.The small‐ and medium‐sized neurons of the caudal portions of the nucleus solitarius are internuncial in position with respect to cardiovascular afferents of the glossopharyngeal and vagus nerves and sympathetic and parasympathetic cardiovascular efferent neurons of the medulla. The results of this study suggest that these3H‐digoxin‐labeled cells, presumably neurons of nucleus solitarius, may possess high affinity binding sites for digoxin. Further, the area postrema, which lacks a blood‐brain barrier, may provide a portal of entry for3H‐digoxin into regions of the medulla known to contain neurons that play a role in the r
机译:摘要本研究的目的是定位大鼠髓质中强心苷地高辛的结合位点。成年雄性 Sprague-Dawley 大鼠被注射 (IV) 3H-地高辛并在 30 分钟后杀死。髓质凛自显影显示 3H-地高辛与孤立核、迷走神经背运动核、后区以及后区和下层神经细胞之间区域的中小型神经细胞结合的证据。然而,背侧运动核的副交感神经节前神经元没有被标记。髓质中3H-地高辛标记的细胞主要位于后区水平的孤核的混合部分和内侧部分。注射未标记的地高辛后注射3H-地高辛的动物显示出放射性结合的减少。孤核尾部的中小型神经元相对于舌咽神经和迷走神经的心血管传入神经以及延髓的交感神经和副交感心血管传出神经元的位置是间位的。这项研究的结果表明,这些3H-地高辛标记的细胞,可能是孤核的神经元,可能具有地高辛的高亲和力结合位点。此外,缺乏血脑屏障的后区可能为3H-地高辛进入已知含有神经元的髓质区域提供入口,这些神经元在髓质中起作用。

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