Significant carbon dioxide evolution in the light could not be betected in maize leaves held in single-leaf chambers. The leaves that were used remained attached to the plant and were kept at 30° C and with 200 w m-2of light incident on the leaf surface. Several experimental techniques were combined in an attempt to detect CO2evolution from cells within the leaf; these techniques included that of passing air through, rather than over, the leaf, using different levels of O2and obtaining CO2response curves
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