A novel immunoenzymatic assay is described for the quantitation of human haptoglobin (Hp). Two binding sites on the Hp molecule, namely for hemoglobin (Hb) and for the specific antibody, are involved in the reaction. Hb adsorbed onto the polystyrene microplate binds Hp present in any biological fluid. The formed Hp-Hb complex is detected with horse-radish peroxidase conjugated with anti-Hp antibody. By means of this ELISA, Hp may be measured in the range of 5 to 150 ug/L. Comparison of the Hp-ELISA with two other methods of Hp determination resulted in correlation coefficients of 0.97 to 0.99. Intra- and inter-assay coefficients of variation ranged from 4.7 to 6.7. Hp levels were measured in urine, cord serum, cerebrospinal fluid, amniotic fluid and saliva.
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