The amino acid composition of TCA-precipitated proteins and of total residues of plant cells grown in suspension culture

摘要

Amino acid analyses were performed on the soluble and total proteins from plant cells grown in suspension culture. The cell cultures originated from 12 different plant species, and the explants were taken from different organs of the plants.Relatively small differences in the amino acid composition existed between the soluble proteins from different species, between cells originating from different organs of the same species, and between the same cultures grown on different media under the same environmental conditions.There was some variation in the proportion of basic, aromatic, and sulfur-containing amino acids which constituted about 17, 9.5, and 3 of the protein amino acids, respectively. The amino acid composition of the soluble proteins of wheat coleoptile and soybean hypocotyl resembled that of the soluble proteins from cultured cells of these plants. Essential amino acids, particularly the basic ones and methionine, were proportionally higher in the cell proteins than those reported for seed proteins.