A Gene Coding for a Zinc Finger Protein Is Induced during 12-O-Tetradecanoylphorbol-13-Acetate-Stimulated HL-60 Cell Differentiation

摘要

ETR103cDNA was cloned as an immediate early gene in the course of macrophagic differentiation of HL-60 cells stimulated by TPA (12-O-tetradecanoylphorbol-13-acetate). The induction by TPA was immediate-early (within 30 min) and transient. This gene was not induced by vitamin D3, or by retinoic acid, which stimulates differentiation of HL-60 cells to the monocytic or granulocytic lineage, respectively. TheETR103mRNA was induced by TPA in lymphoid or myeloid leukemia cell lines of several maturation stages. The induction by TPA seems to proceed by a protein kinase C-mediated mechanism, on the basis of the results obtained by using protein kinase C inhibitor (H-7), protein kinase C activator (diC8), and an activator of protein kinase A (dibutyryl cAMP). Okadaic acid, an inhibitor of protein phosphatases, also induced theETR103mRNA expression. The nucleotide sequence of theETR103cDNA reveals thatETR103encodes a human zinc finger-containing transcription factor identical toEgr-1and225, which is homologous to mouseEgr-1,Zif/268,Krox-24, andTIS8, or to ratNGFI-A.