Study of the idiotypy of lipopolysaccharide‐specific polyclonal and monoclonal antibodies

摘要

AbstractThe monoclonal antibodies produced by a variety of hybridomas making antibody specific forE. coli0113 lipopolysaccharide (LPS) were purified by affinity chromatography and their fine specificity studied. All reacted specifically with the polysaccharide moiety of LPS fromE. coli0113 and fromNeisseria lactamica;two reacted with LPS fromPseudomonas aeruginosaand one reacted with LPS fromKlebsiella pneumoniae.Polyclonal and monoclonal syngeneic and semi‐syngeneic anti‐idiotypic antisera were produced to study the idiotypy of LPS‐specific monoclonal antibodies which express a complex cross‐reactive idiotype (IdX) as well as individual idiotypes.E. coli0113 LPS‐specific antibodies produced by B ALB/c mice express this IdX and the kinetics for its expression was examined using mice either primed or hyperim‐munized with LPS; idiotypic maturation was observed, but we were unable to detect an auto‐anti‐idiotypic antibody response. This IdX was expressed onE. coli0113 LPS‐specific antibodies from all strains of mice examined, indicating that its expression is not restricted by genes linked