A Micromethod for the Detection of TsubG/suband TsubM/subCells. Technical Aspects for the Enumeration of Human T-Cell Subpopulations

摘要

A rosette technique for the detection of human T-lymphocyte subpopulations with Fc receptors for IgG (TG cells) or IgM (Tm cells) has been worked out using minimal amounts of IgG and IgM antibodies. Antibody-coated ox red blood cells (EA) were used as indicator cells. Optimal conditions for rosette enumerations required intimate T-cell-EA contact and incubation of cell mixtures for 3 h at 4°C. The TM-cell level increased after culture of T cells, and TM-cell rosettes were found to be more fragile. The rosette assay was found to be reproducible and thus useful in clinical immunology