Evidence that the Auberger Blood Group Antigens Are Located on the Lutheran Glycoproteins

摘要

Abstract.Immunoblots of red cell membranes stained with eluates of alloanti‐Auaand alloanti‐Aubshow that these antibodies recognize 2 membrane components from Au(a+) and Au(b+) cells, respectively. These structures, of apparent molecular weight (Mr) 79,000 and 85,000, are identical in appearance and mobility on a 10 SDS polyacrylamide gel to the Lutheran glycoproteins identified by alloanti‐Lub. Like the Lutheran glycoproteins, they showed a reduction in apparent Mrof about 1,500 after sialidase treatment. Red cell membrane components immunoprecipitated by a Lutheran‐related monoclonal antibody, were analysed with Lutheran and Auberger antibodies by immunoblotting. The Lutheran glycoproteins were revealed by anti‐Lubin precipitates from Au(a+b‐) Lu(a‐b+) and Au(a‐b+) Lu(a‐b+) cells, by anti‐Auain precipitates from Au(a+b‐) Lu(a‐b+) cells and by anti‐Aubin precipitates from Au(a‐b+) Lu(a‐b+) cells. The same components were also recognized by anti‐Luain precipitates from Lu(a+b‐) cells. Thus Auaand Aubantigens appear to be carried on the same red cell membrane structures as those carrying the Lutheran determinants. These results are particularly significant in the light of the very close phenotypic association between the Auberger and Lutheran blood groups which have been shown, by one family, to be con