A simple and sensitive method is described for the determination of chlormethiazole in blood. Separation and quantitation were performed by reversed-phase high performance liquid chromatography utilizing ultraviolet detection at 254 nm. The internal standard, 2-amino-4-methylthiazole, was used as an aid to quantitation. The blood sample was deproteinized with phosphotungstic acid reagent and the clear supernatant obtained was directly analysed in the chromatographic system. Limit of detection was about 0.25 #x3BC;g/ml blood. Recovery of chlormethiazole from blood was 73 #xB1; 2. The mean between-run coefficient of variation was 4.9 over the range of 0.25-32.0 #x3BC;g/ml, whereas the within-run coefficient of variation at 0.25 #x3BC;g/ml was 6.5. The scale and sensitivity of detection were found to be suitable for use in pharmacokinetic studies in the rat.
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