The effects of in vivo and in vitro treatment of immunocompetent cells with heterologous antiserum IgG were studied using the LPT technique and isogeneic strains of guinea pigs. Treatment of hypersensitive animals with ALS, AMS and ATS was shown to effect suppression of delayed-type cutaneous hypersensitivity to SRBC confirming the findings presented in the previous study. Treatment of normal isogeneic recipients with ALS, AMS and ATS likewise induced significant suppression of LPT reactions induced in these animals with cells from donors hypersensitized with SRBC. However, no suppression of LPT reactions was detectable using: (1) PE cells from hypersensitized donors treated with immune IgG and in which suppression was demonstrable prior to harvest of cells or (2) PE cells from hypersensitive donors treated in vitro with immune IgG prior to LPT. These findings suggest that the primary mechanism whereby heterologous anti-serum IgG induces suppression involves impairment of the capacity of cells to migrate.
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