Detection of α S1-casein in vomit from bottle-fed babies by enzyme-linked immunosorbent assay

摘要

This study describes a sensitive enzyme-linked immunosorbent assay (ELISA) using rabbit anti-bovine α S1-casein antibody for the detection of commercial milk and milk-containing vomit. The antibody does not react with other human body fluids such as breast milk. The stability of α S1-casein antigenic activity was examined after storage at different temperatures and enzyme digestion. There was no decrease after storage for one year at room temperature but 40 of the activity was lost after 6 months at 37°C. Enzyme digestion (6 hours, 37°C) resulted in 65 ∼ 70 loss of activity but the antibody reacted with the peptide fragments of α S1-casein. Vomit samples from 3 normal infants were tested by ELISA, and a S 1-casein could be detected in 1 cm