Analysis of chromosomal DNA patterns of the genusKluyveromyces

摘要

AbstractUsing an improved procedure of pulsed field gel electrophoresis, yeast chromosomes were separated over a wide range of molecular size (250–4000 kbp) on single gels. The chromosomal DNA patterns of all the species belonging to the genusKluyveromyceswere examined. Within the speciesK. marxianus, the varietieslactis,drosophilarumandvanudeniishowed closely related patterns; very different from them, the varietiesbulgaricusandmarxianuswere related to each other, forming a distinct group; the strains commonly called ‘K. lactis’ and ‘K. fragilis’ were unambiguously different from each other in chromosome patterns. These differences were correlated with the presence of characteristic repetitive sequence elements in the mitochondrial DNA of the former group and not in the latter. Analysis ofCandida macedoniensis, which had been considered to be an anamorph ofK. marxianusvar.marxianus, showed that these two yeast species were indeed similar in chromosome patterns and in mitochondrial DNA restriction