Effects of the Platelet-Activating Factor Antagonist BN 52021 on Anti-Islet Cytotoxicity of Mononuclear Cells and Serum from Type 1 (Insulin-Dependent) Diabetic Patients

摘要

The effect of platelet-activating factor (PAF) antagonist BN 52021 (0.06–2.5 mM) on the cytotoxic activity of mononuclear cells (MNC) from newly diagnosed type 1 diabetic patients against 51Cr-labeled Langerhans islets from neonatal rats was investigated in a 6-hour cytotoxicity test. A dose-dependent inhibition of anti-islet cytotoxicity by BN 52021 was observed. The suppression of the islet lysis was significant at a concentration of 0.6 mM BN 52021. During a 4-day cell culture, BN 52021 had no inhibitory effect on the antigen-mediated triggering of immunocytes with anti-islet cytotoxicity. The results suggest that the drug is only effective during immunocytolytic reactions of MNC against pancreatic islets. A PAF-independent action of BN 52021 can not be excluded at presen