Demonstration of glycosaminoglycans in Caenorhabditis elegans

摘要

A considerable amount (approximately 1.6 mu;g from 1 mg of dried nematode) of non-sulfated chondroitin, two orders of magnitude less yet an appreciable amount of heparan sulfate, and no hyaluronate were found in Caenorhabditis elegans nematodes. The chondroitin chains were heterogeneous in size, being shorter than that of whale cartilage chondroitin sulfate. The disaccharide composition analysis of heparan sulfate revealed diverse sulfation including glucosamine 2- N -sulfation, glucosamine 6- O -sulfation and uronate 2- O -sulfation. These results imply that chondroitin and heparan sulfate are involved in fundamental biological processes.