As the genome of B. mori is available in GenBank and the EST database of B. mori is expanding, identification of novel genes of B. mori was conceivable by data-mining techniques and bioinformatics tools. In this study, we used the in silico cloning method to identify eukaryotic initiation factor 5A (eIF5A) gene in B. mori. With the hypusine formation, eIF5A is involved in the regulation of cell proliferation and apoptosis. Using the computer program MEGA3, we conducted a search for homologs of eIF5A among many eukaryotic species and confirmed that the eIF5A was conserved in all organisms investigated. This gene has been registered in GenBank under the accession number DQ104412.
展开▼
机译:由于 GenBank 中已有 B. mori 的基因组,并且 B. mori 的 EST 数据库正在扩大,因此可以通过数据挖掘技术和生物信息学工具鉴定出 B. mori 的新基因。本研究采用计算机克隆方法鉴定了森双歧杆菌的真核起始因子5A(eIF5A)基因。随着hypusine的形成,eIF5A参与细胞增殖和凋亡的调节。使用计算机程序 MEGA3,我们在许多真核生物物种中搜索了 eIF5A 的同源物,并确认 eIF5A 在所有研究的生物体中都是保守的。该基因已在GenBank中注册,登录号为DQ104412。
展开▼