Separation of Eicosanoids by Reverse-Phase Thin-Layer Chromatography

摘要

A method is described for separating eicosanoids including prostaglandins, their metabolites, and HETE. This was accomplished using commercially available reverse-phase thin-layer chromatographic plates and 0.0025M phosphoric acid-acetonitrile (52:48, v/v) containing 0.2M sodium chloride as a mobile phase. The use of reverse-phase thin-layer chromatography offers an alternative means of separating eicosanoids over traditional silica gel.